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SRT-C

SRT-C

SRT®-C SEC phase, developed based on innovative surface coating technology, comprises of uniform, hydrophilic, and neutral nanometer thick film chemically bonded to high purity and mechanically stabilized silica. SRT®-C, uses lay-down monolayer on porous silica as oppose to SRT of stand-up monolayer (as shown below), offers ideal phase chemistry for hydrophobic samples like insulin, membrane protein monoclonal antibodies derivatized with polymer branches (polypeptide or PEG) separation.

 

  • Description
  • Characteristics
  • Applications/Downloads
Phase    SRT®-C SEC-150 SRT®-C SEC-300 SRT®-C SEC-500
Material    Neutral, hydrophilic film bonded silica
Particle size    5 µm
Pore size        ~ 150 Å ~ 300 Å ~ 500 Å
Protein MW range (native)   500 - 150,000 5,000 - 1,250,000 15,000 - 5,000,000
pH stability   2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure (for a 7.8×300 mm)   ~ 700 psi ~ 700 psi ~ 700 psi
Maximum backpressure   ~ 4,500 psi ~ 3,500 psi ~ 3,000 psi
Salt concentration range   20 mM - 2.0 M
Maximum temperature   ~ 80 °C
Mobile phase compatibility   Aqueous and organic
  • Ideal for separation and analysis of hydrophobic proteins, and monoclonal antibodies derivatized with polymer branches such as polypeptide or PEG
  • Particle size of 5 µm
  • Selection of pore size: 100, 150, 300, 500, 1,000, and 2,000 Å
  • High separation efficiency and resolution
  • High capacity
  • High lot-to-lot reproducibility
  • High protein recovery with intact biological activity
  • Negligible non-specific interactions

 

Catalog User Manual

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Part Number Description QTY
235100P-4630 SRT-C SEC-100 PEEK , 5um, 100 A 4.6 x 300 mm

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235100P-4605 SRT-C SEC-100 PEEK , 5um, 100 A 4.6 x 50 mm

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235100-4615 SRT-C SEC-100, 5um, 100 A 4.6 x 150 mm

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235100-4625 SRT-C SEC-100, 5um, 100 A 4.6 x 250 mm

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235100-4630 SRT-C SEC-100, 5um, 100 A 4.6 x 300 mm

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235100-4605 SRT-C SEC-100, 5um, 100 A 4.6 x 50 mm

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235100-7815 SRT-C SEC-100, 5um, 100 A 7.8 x 150 mm

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235100-7825 SRT-C SEC-100, 5um, 100 A 7.8 x 250 mm

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235100-7830 SRT-C SEC-100, 5um, 100 A 7.8 x 300 mm

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235100-7805 SRT-C SEC-100, 5um, 100 A 7.8 x 50 mm

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