High-performance liquid chromatography and its derivatives techniques have become the dominant analytical separation tools in the pharmaceutical, chemical and food indundstries, environmental laboratories, and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now no singal book has focused on the theory and practice of column technology.
The Atlantis family of HPLC columns was created to solve one the most chromatographic problems, retaining polar compunds. Through historical experience and synthetic innovation, Atlantis columns lead the industry in porviding exceptional performance, versatility, retention and chromatographic stability for polar compounds, while also affording balanced retention for broad analtye mixtures. For retention and separation of polar compunds via reversed-phase HPLC, Atlantis T3 columns set the standard for polar compound retention. Atlantis T3 columns are a universal, silica-based, reversed-phase C18 line of HPLC columns that not only retin and separate small, water-soluble polar organic compunds, but also provide superior performance accross a wider pH range. Atlantis columns are the culmination of over 30 years of bonded phase research and are a vast improvement upon the highly successful Atlantis dC18 line of columns. For reversed-phase application, Atlantis T3 columns should be considered the first choice when developing a separation of polar (and non-polar) compounds. The advantages of T3 bonding technology is extended to UPLC in the Acquity UPLC HSS T3 columns.
SunFire columns set the standard for the state-of-the-art bonded C19 and C8 silica HPLC Columns. Benefiting from years of research and product development, SunFire columns represent the best in particle and bonding expertise and deliver the industry-leading level of chromatogrpahic performance.
The universal acceptance by pharmaceutical scientists in drug discovery, method development, and isolation and purification has enabled XTerra columns to become the fastest selling and most successful column product in the world of chromatography. The reason : the enabling science of Hybrid Particle Technology. Drug discovery scientists benefit from the DMSO injection-resistance of the rugged XTerra particle. Method development scientists are able to operate at whatever pH is necessary for optimal selectivity due to XTerra columns' wide pH range. Isolation and purification scientists can load up to 60x more material per injection because of the high mass loading capacity of XTerra columns.
As today's chemists establish new analyical methods for the latest pharmaceutical and biopharmaceutical products, the selection of a reproducible HPLC column is essential. The selected column needs to provide th same chromatographic results over the life of the new drug product. Symmetry columns demonstrate superior reproducibility over many years. Batches randomly selected over a five year period demonstrate excellent reproducibility. Reproducibility is our number one priority in supplying symmetry columns. This excellent reproducibility is a result of our commitment to maintainting the tightest specifications in the HPLC column industry. Symmetry columns are engineered with high purity raw materials, tightly controlled manufacturing pro cesses and column packing procedures that provide today's scientists with the best, most reproducible HPLC column available. Our goal is the supply a family of HPLC columns that you can rely on for rugged and robust methods. Symmetry columns let you increase your laboratories productivity and allow easier method transfer between labs and around the globe.
One of the great advantages of choosing Waters Spherisorb columns is the range of choices available to you. Waters Spherisorb columns are produced in a wide range of sizes and bonded phases to meet your chrmatographic needs. Silica is produced in 2, 5 and 10 micron particle sizes. The high quality bonded phases ive you many different and unique separation selectives.
The 4um small particle size of a Nova-Pak bonded phases offers high resolution as well as more efficient and faster chromatography. This is accomplished by using shorter columns, thereby reducing solvent consumption or by using longer columns to resolve even the most complex mixture. Analytical columns with a 4um particle size packings are available in 75, 150 and 300mm length steel columns. Steel cartridge columns with reusable endfittings are available in 50, 100, 150 and 250mm lengths. Semi-preparative columnns with 6 micron particle size packings give you an unparalled range of separation possibilities. Stringent QC procedures in our cGMP manufacturing facility ensure batch-to-batch reproducibilty.
If your method calls for µBondapak C18 packings, there is only one µBondapak C18 packing material. Almost anything can be made once, but making a packing material reproducible year-to-year for over 30 years demostrates true manufacturing and QC capability. Many companies clain to have columns with "µBondapak-like selectivity" but not one has ever passed waters stringent QC batch test. Waters has set the standard for reproducible, high-performance chomatographic columns. Waters µBondapak columns are still the most widely referenced and requested columns un the world.