Chromex Scientific > Products > HPLC Products > HPLC Columns > Waters

Go Straight to Ordering Information

The Atlantis family of HPLC columns was created to solve one the most chromatographic problems, retaining polar compunds. Through historical experience and synthetic innovation, Atlantis columns lead the industry in porviding exceptional performance, versatility, retention and chromatographic stability for polar compounds, while also affording balanced retention for broad analtye mixtures. For retention and separation of polar compunds via reversed-phase HPLC, Atlantis T3 columns set the standard for polar compound retention. Atlantis T3 columns are a universal, silica-based, reversed-phase C18 line of HPLC columns that not only retin and separate small, water-soluble polar organic compunds, but also provide superior performance accross a wider pH range. Atlantis columns are the culmination of over 30 years of bonded phase research and are a vast improvement upon the highly successful Atlantis dC18 line of columns. For reversed-phase application, Atlantis T3 columns should be considered the first choice when developing a separation of polar (and non-polar) compounds. The advantages of T3 bonding technology is extended to UPLC in the Acquity UPLC HSS T3 columns.

T3 bonding

The superior performance of Atlantis T3 HPLC columns and the ACQUITY UPLC HSS T3 columns is a result of Waters advanced T3 bonding process. T3 bonding utilizes a trifunctional C18 alkyl phase bonded at a liquid density that promotes polar compound retention and aqueous mobile-phase compatibility. The proprietary T3 endcapping process is much more effective than traditional trimethly-silane (TMS) endcapping.

This unique combination of bonding and endcapping provided superior polar compound retention and aqueous compatibility while also enhancing column performance, lifetime, peak shape and stability.

Long Column Lifetimes at Low pH

The creation of highly retentive, reversed-phase C18 columns for polar compound retention involves bonding at a ligand density that is less hydrophobic and, therefore, more compatible with the weak, highly aqueous mobile phases required for retaining polar compounds. When exposed to strongly acidic mobile phases (i.e.

Atlantis T3 columns resist ligand cleavage by utilizing a trifunctional attachment of the C18 phase to the particle surface, thus providing exceptional column life at low pH.

Improved pH 7 Performance

At pH 7, poor peak shape for amine-containing bases and shortened column lifetimes are encountered when using intermediate ligand density C18 columns designed for polar compound retention. Poor peak shape is due to secondary interactions with unreacted silanols that remain present after bonding and endcapping. The proprietary T3 endcapping procedure reacts with more of these active silanols thereby dramatically improving peak shape for bases. Shortened column lifetimes are due to the dissolution of the silica particle substrate by the high pH mobile phases. The more effective and efficient T3 bonding helps “protect” the silica substrate from dissolution, thus providing longer column lifetime.

Balanced Retention of diverse Analyte mixtures

When faced with the task of analysing a number of compounds encompassing a broad range of popularity, few options exist that will result in adequate retention of polar species without excessive retention of the hydrophic components. In drug discovery, a large number of candidates need to be screened, often with little or no characterisation of their chemical properties. Many polar candidates are often overlooked due to their elution in the void space of the column, while hydrophobic species may be permanently bound to the stationary phase. Atlantis T3 columns are designed to deliver exceptional performance for an expansive range of compound polarities, thus being ideally suited as a universal reversed-phase column for open environments.

ACQUITY UPLC HSS T3 Columns for Polar Compound Retention

ACQUITY UPLC HSS (High Strength Silica) columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000psi/1000bar. The ACQUITY UPLC HSS particle is not an HPLC particle. High pore volume HPLC particle do not possess the operating pressures of UPLC separations. The first ligand chemistry in this new UPLC-certified column family utilises T3 bonding in order t retain and separate polar organic compounds. ACQUITY UPLC HSS T3 columns possess the superior polar-compound retention, aqueous mobile-phase compatibility and ultra-low MS bleed of Atlantis T3 columns.

Easier Method Transfer

Since Atlantis T3 HPLC and ACQUITY UPLC BEH columns is nearly seamless, as the hybrid particle substrates differ only in particle size. However, because of T3 bonding it should be straightforward to make simple adjustments to successfully transfer a separation from an Atlantis T3 HPLC column to an ACQUITY UPLC HSS T3 column.

Atlantis HILIC Silica

Hydrophilic-Interaction Chromatography (HILIC)

HILIC is an alternative chromatographic technique that offers complementary selectivity to RP and often retains very polar species that cannot be retained by traditional means. Atlantis HILIC Silica columns were designed to retain very polar, organic molecules that are too polar to retain by RP. Unlike the highly aqueous mobile phases required for polar retention in RP separations. Atlantis HILIC Silica columns employ highly volatile (>80% organic) mobile phases which are ideal for mass spectrometry (MS) response and sensitivity. Additionally, direct compatibility with high organic SPE elutes dramatically increases the number of samples the can be handled, thus substantially increasing sample throughput. HILIC retention mechanisms are a complex combination of partitioning, ion-exchange and hydrogen bonding, resulting in enhanced retention for polar analytes. Atlantis HILIC Silica columns, used in combination with high organic (>80% acetonitrile) mobile phases, result in retention of analytes that are simply too polar to retain by traditional RP chromatography.

Enhanced Mass Spectrometry Response

Utilisation of HILIC has grown in popularity, primarily due to the extensive adoption of mass spectrometry as a detector and the necessity of improving sensitivity for the quantitation of polar analytes. Unlike reversed-phase which utilises high aqueous mobile phases to induce retention, HILIC employs an aceteonitrile-rich mobile phase. This high organic mobile phase is easily deslovated, resulting in improved ionisation efficiency and mass spectrometry response. For these reasons, HILIC has become a popular technique for bioanalytical laboratories.

Ordering Information

Part No. Description Price

186003715 Atlantis dT3 3 μm 2.1 x 20 mm; Enquire

Part No. Description dc18 Price

186001279 Atlantis dC18 3 μm 1 x 50 mm; Enquire

186001283 Atlantis dC18 3 μm 1 x 150 mm; Enquire

186001287 Atlantis dC18 3 μm 2.1 x 30 mm; Enquire

186001291 Atlantis dC18 3 μm 2.1 x 50 mm; Enquire

186001295 Atlantis dC18 3 μm 2.1 x 100 mm; Enquire

186001299 Atlantis dC18 3 μm 2.1 x 150 mm; Enquire

186001303 Atlantis dC18 3 μm 3 x 100 mm; Enquire

186001307 Atlantis dC18 3 μm 3 x 150 mm; Enquire

186001317 Atlantis dC18 3 μm 3.9 x 150 mm; Enquire

186001325 Atlantis dC18 3 μm 4.6 x 30 mm; Enquire

186001329 Atlantis dC18 3 μm 4.6 x 50 mm; Enquire

186001333 Atlantis dC18 3 μm 4.6 x 75 mm; Enquire

186001337 Atlantis dC18 3 μm 4.6 x 100 mm; Enquire

186001342 Atlantis dC18 3 μm 4.6 x 150 mm; Enquire

186001385 Atlantis dC18 3 μm 3.9 x 50 mm; Enquire

186001389 Atlantis dC18 3 μm 3 x 50 mm; Enquire

186001393 Atlantis dC18 3 μm 3.9 x 100 mm; Enquire

186002058 Atlantis dC18 3 μm 2.1 x 20 mm; Enquire

186002060 Atlantis dC18 3 μm 3 x 20 mm; Enquire

186002062 Atlantis dC18 3 μm 4.6 x 20 mm; Enquire

186002064 Atlantis dC18 3 μm 2.1 x 15 mm Enquire

186002312 Atlantis dC18 3 μm 4.6 x 150 mm; Enquire

186001313 Atlantis dC18 3 μm 3.9 x 20 mm; Enquire

186001321 Atlantis dC18 3 μm 4.6 x 20 mm; Enquire

186001377 Atlantis dC18 3 μm 2.1 x 10 mm; Enquire

186001381 Atlantis dC18 3 μm 2.1 x 20 mm; Enquire

186001281 Atlantis dC18 5 μm 1 x 50 mm; Enquire

186001285 Atlantis dC18 5 μm 1 x 150 mm; Enquire

186001289 Atlantis dC18 5 μm 2.1 x 30 mm; Enquire

186001293 Atlantis dC18 5 μm 2.1 x 50 mm; Enquire

186001297 Atlantis dC18 5 μm 2.1 x 100 mm; Enquire

186001301 Atlantis dC18 5 μm 2.1 x 150 mm; Enquire

186001305 Atlantis dC18 5 μm 3 x 100 mm; Enquire

186001309 Atlantis dC18 5 μm 3 x 150 mm; Enquire

186001311 Atlantis dC18 5 μm 3 x 250 mm; Enquire

186001319 Atlantis dC18 5 μm 3.9 x 150 mm; Enquire

186001327 Atlantis dC18 5 μm 4.6 x 30 mm; Enquire

186001331 Atlantis dC18 5 μm 4.6 x 50 mm; Enquire

186001335 Atlantis dC18 5 μm 4.6 x 75 mm; Enquire

186001340 Atlantis dC18 5 μm 4.6 x 100 mm; Enquire

186001344 Atlantis dC18 5 μm 4.6 x 150 mm; Enquire

186001346 Atlantis dC18 5 μm 4.6 x 250 mm; Enquire

186001361 Atlantis dC18 5 μm 19 x 10 mm; Enquire

186001387 Atlantis dC18 5 μm 3.9 x 50 mm; Enquire

186001391 Atlantis dC18 5 μm 3 x 50 mm; Enquire

186001395 Atlantis dC18 5 μm 3.9 x 100 mm; Enquire

186002059 Atlantis dC18 5 μm 2.1 x 20 mm; Enquire

186002061 Atlantis dC18 5 μm 3 x 20 mm; Enquire

186002063 Atlantis dC18 5 μm 4.6 x 20 mm; Enquire

186002065 Atlantis dC18 5 μm 2.1 x 15 mm; Enquire

186002298 Atlantis dC18 5 μm 10 x 50 mm; Enquire

186002299 Atlantis dC18 5 μm 10 x 100 mm; Enquire

186002300 Atlantis dC18 5 μm 10 x 10 mm; Enquire

186002311 Atlantis dC18 5 μm 4.6 x 150 mm; Enquire

186002313 Atlantis dC18 5 μm 4.6 x 250 mm; Enquire

186001315 Atlantis dC18 5 μm 3.9 x 20 mm; Enquire

186001323 Atlantis dC18 5 μm 4.6 x 20 mm; Enquire

186001379 Atlantis dC18 5 μm 2.1 x 10 mm; Enquire

186001383 Atlantis dC18 5 μm 2.1 x 20 mm; Enquire

186001363 Atlantis dC18 10 μm 19 x 10 mm; Enquire

186002452 Atlantis dC18 10 μm 10 x 10 mm; Enquire

186002453 Atlantis dC18 10 μm 10 x 150 mm; Enquire

186002454 Atlantis dC18 10 μm 10 x 250 mm; Enquire

186004030 Atlantis dC18 5 μm 19 x 250 mm; Enquire

Part No. Description hilic Price

186002003 Atlantis HILIC 3 μm 1 x 50 mm; Enquire

186002009 Atlantis HILIC 3 μm 2.1 x 30 mm; Enquire

186002011 Atlantis HILIC 3 μm 2.1 x 50 mm; Enquire

186002013 Atlantis HILIC 3 μm 2.1 x 100 mm; Enquire

186002015 Atlantis HILIC 3 μm 2.1 x 150 mm; Enquire

186002017 Atlantis HILIC 3 μm 3 x 50 mm; Enquire

186002019 Atlantis HILIC 3 μm 3 x 100 mm; Enquire

186002025 Atlantis HILIC 3 μm 4.6 x 30 mm; Enquire

186002027 Atlantis HILIC 3 μm 4.6 x 50 mm; Enquire

186002029 Atlantis HILIC 3 μm 4.6 x 100 mm; Enquire

186002031 Atlantis HILIC 3 μm 4.6 x 150 mm; Enquire

186002005 Atlantis HILIC 3 μm 2.1 x 10 mm; Enquire

186002021 Atlantis HILIC 3 μm 3.9 x 20 mm; Enquire

186002023 Atlantis HILIC 3 μm 4.6 x 20 mm; Enquire

186002007 Atlantis HILIC 3 μm 2.1 x 15 mm; Enquire

186002315 Atlantis HILIC 3 μm 4.6 x 150 mm; Enquire

186002004 Atlantis HILIC 5 μm 1 x 50 mm; Enquire

186002010 Atlantis HILIC 5 μm 2.1 x 30 mm; Enquire

186002012 Atlantis HILIC 5 μm 2.1 x 50 mm; Enquire

186002014 Atlantis HILIC 5 μm 2.1 x 100 mm; Enquire

186002016 Atlantis HILIC 5 μm 2.1 x 150 mm; Enquire

186002018 Atlantis HILIC 5 μm 3 x 50 mm; Enquire

186002020 Atlantis HILIC 5 μm 3 x 100 mm; Enquire

186002026 Atlantis HILIC 5 μm 4.6 x 30 mm; Enquire

186002028 Atlantis HILIC 5 μm 4.6 x 50 mm; Enquire

186002030 Atlantis HILIC 5 μm 4.6 x 100 mm; Enquire

186002032 Atlantis HILIC 5 μm 4.6 x 150 mm; Enquire

186002033 Atlantis HILIC 5 μm 4.6 x 250 mm; Enquire

186003959 Atlantis HILIC 5 μm 19 x 150 mm; Enquire

186003960 Atlantis HILIC 5 μm 30 x 50 mm; Enquire

186003961 Atlantis HILIC 5 μm 30 x 100 mm; Enquire

186003962 Atlantis HILIC 5 μm 30 x 150 mm; Enquire

186002006 Atlantis HILIC 5 μm 2.1 x 10 mm; Enquire

186002022 Atlantis HILIC 5 μm 3.9 x 20 mm; Enquire

186002024 Atlantis HILIC 5 μm 4.6 x 20 mm; Enquire

186003956 Atlantis HILIC 5 μm 19 x 10 mm; Enquire

186003957 Atlantis HILIC 5 μm 19 x 50 mm; Enquire

186003958 Atlantis HILIC 5 μm 19 x 100 mm; Enquire

186002008 Atlantis HILIC 5 μm 2.1 x 15 mm; Enquire

186002314 Atlantis HILIC 5 μm 4.6 x 150 mm; Enquire

186002316 Atlantis HILIC 5 μm 4.6 x 250 mm; Enquire

Part No. Description obd Price

186001365 Atlantis OBD 5 μm 19 x 50 mm; Enquire

186001367 Atlantis OBD 5 μm 19 x 100 mm; Enquire

186001373 Atlantis OBD 5 μm 30 x 50 mm; Enquire

186001375 Atlantis OBD 5 μm 30 x 100 mm; Enquire

186002455 Atlantis OBD 5 μm 30 x 75 mm; Enquire

186002800 Atlantis OBD 5 μm 19 x 150 mm; Enquire

186002801 Atlantis OBD 5 μm 30 x 150 mm; Enquire

186003696 Atlantis OBD 5 μm 19 x 50 mm; Enquire

186003697 Atlantis OBD 5 μm 19 x 100 mm; Enquire

186003698 Atlantis OBD 5 μm 19 x 150 mm; Enquire

186003700 Atlantis OBD 5 μm 30 x 50 mm; Enquire

186003701 Atlantis OBD 5 μm 30 x 75 mm; Enquire

186003702 Atlantis OBD 5 μm 30 x 100 mm; Enquire

186003703 Atlantis OBD 5 μm 30 x 150 mm; Enquire

186001369 Atlantis OBD 10 μm 19 x 150 mm; Enquire

186001371 Atlantis OBD 10 μm 19 x 250 mm; Enquire

186002417 Atlantis OBD 10 μm 30 x 150 mm; Enquire

186002418 Atlantis OBD 10 μm 30 x 250 mm; Enquire

186003707 Atlantis OBD 10 μm 19 x 50 mm; Enquire

186003708 Atlantis OBD 10 μm 19 x 150 mm; Enquire

186003709 Atlantis OBD 10 μm 19 x 150 mm; Enquire

186003711 Atlantis OBD 10 μm 30 x 150 mm; Enquire

186003712 Atlantis OBD 10 μm 30 x 250 mm; Enquire

Part No. Description prep Price

186003691 Atlantis Prep 5 μm 10 x 50 mm; Enquire

186003692 Atlantis Prep 5 μm 10 x 100 mm; Enquire

186003693 Atlantis Prep 5 μm 10 x 150 mm; Enquire

186003694 Atlantis Prep 5 μm 10 x 250 mm; Enquire

186003695 Atlantis Prep 5 μm 10 x 10 mm; Enquire

186003699 Atlantis Prep 5 μm 19 x 10 mm; Enquire

186004026 Atlantis Prep 5 μm 19 x 250 mm; Enquire

186004080 Atlantis Prep 5 μm 50 x 50 mm; Enquire

186004081 Atlantis Prep 5 μm 50 x 100 mm; Enquire

186004082 Atlantis Prep 5 μm 50 x 150 mm; Enquire

186003704 Atlantis Prep 10 μm 10 x 150 mm; Enquire

186003705 Atlantis Prep 10 μm 10 x 250 mm; Enquire

186003706 Atlantis Prep 10 μm 10 x 10 mm; Enquire

186003710 Atlantis Prep 10 μm 19 x 10 mm; Enquire

186004083 Atlantis Prep 10 μm 50 x 50 mm; Enquire

186004084 Atlantis Prep 10 μm 50 x 100 mm; Enquire

186004085 Atlantis Prep 10 μm 50 x 150 mm; Enquire

186004086 Atlantis Prep 10 μm 50 x 250 mm; Enquire

186004712 Atlantis Prep 10 μm 30 x 75 mm; Enquire

Part No. Description t3 Price

186003713 Atlantis T3 3 μm 1 x 50 mm; Enquire

186003714 Atlantis T3 3 μm 1 x 150 mm; Enquire

186003716 Atlantis T3 3 μm 2.1 x 30 mm; Enquire

186003717 Atlantis T3 3 μm 2.1 x 50 mm; Enquire

186003718 Atlantis T3 3 μm 2.1 x 100 mm; Enquire

186003719 Atlantis T3 3 μm 2.1 x 150 mm; Enquire

186003720 Atlantis T3 3 μm 3 x 20 mm; Enquire

186003721 Atlantis T3 3 μm 3 x 50 mm; Enquire

186003722 Atlantis T3 3 μm 3 x 100 mm; Enquire

186003723 Atlantis T3 3 μm 3 x 150 mm; Enquire

186003724 Atlantis T3 3 μm 4.6 x 20 mm; Enquire

186003725 Atlantis T3 3 μm 4.6 x 30 mm; Enquire

186003726 Atlantis T3 3 μm 4.6 x 50 mm; Enquire

186003727 Atlantis T3 3 μm 4.6 x 75 mm; Enquire

186003728 Atlantis T3 3 μm 4.6 x 100 mm; Enquire

186003729 Atlantis T3 3 μm 4.6 x 150 mm; Enquire

186003730 Atlantis T3 5 μm 1 x 50 mm; Enquire

186003731 Atlantis T3 5 μm 1 x 150 mm; Enquire

186003732 Atlantis T3 5 μm 2.1 x 20 mm; Enquire

186003733 Atlantis T3 5 μm 2.1 x 30 mm; Enquire

186003734 Atlantis T3 5 μm 2.1 x 50 mm; Enquire

186003735 Atlantis T3 5 μm 2.1 x 100 mm; Enquire

186003736 Atlantis T3 5 μm 2.1 x 150 mm; Enquire

186003737 Atlantis T3 5 μm 3 x 20 mm; Enquire

186003738 Atlantis T3 5 μm 3 x 50 mm; Enquire

186003739 Atlantis T3 5 μm 3 x 100 mm; Enquire

186003740 Atlantis T3 5 μm 3 x 150 mm; Enquire

186003741 Atlantis T3 5 μm 3 x 250 mm; Enquire

186003742 Atlantis T3 5 μm 4.6 x 20 mm; Enquire

186003743 Atlantis T3 5 μm 4.6 x 30 mm; Enquire

186003744 Atlantis T3 5 μm 4.6 x 50 mm; Enquire

186003745 Atlantis T3 5 μm 4.6 x 75 mm; Enquire

186003746 Atlantis T3 5 μm 4.6 x 100 mm; Enquire

186003747 Atlantis T3 5 μm 4.6 x 150 mm; Enquire

186003748 Atlantis T3 5 μm 4.6 x 250 mm; Enquire

186003756 Atlantis T3 3 μm 2.1 x 10 mm; Enquire

186003757 Atlantis T3 3 μm 3.9 x 20 mm; Enquire

186003758 Atlantis T3 3 μm 4.6 x 20 mm; Enquire

186003759 Atlantis T3 5 μm 2.1 x 20 mm; Enquire

186003760 Atlantis T3 5 μm 3.9 x 20 mm; Enquire

186003761 Atlantis T3 5 μm 4.6 x 20 mm; Enquire

186003751 Atlantis T3 3 μm 4.6 x 150 mm; Enquire

186003754 Atlantis T3 5 μm 4.6 x 150 mm; Enquire

186003755 Atlantis T3 5 μm 4.6 x 150 mm; Enquire

Back to the Top of the Page