 |
Carbomix
Carbomix lines of columns have been specifically designed for high resolution separation of water soluble and partially water soluble organic compounds, including carbohydrates, organic acids, peptides, and small bio organic molecules involved in cell metabolism. These novel packing materials are based on low crosslinked (5%, 8% and 10%) polystyrene/divinylbenzene (PS/DVB) particles (5 and 10 mm) with surface modification of sulfonic acid (-SO3H) for Carbomix H-NP resins, followed by chelating of various metal ions, calcium ions (Ca2+) for Carbomix Ca-NP, lead ion (Pb2+) for Carbomix Pb-NP, potassium ion (K+) for Carbomix K-NP, and sodium ion (Na+) for Carbomix Na-NP resins (Figure 1).
Uniform Particle size. The particle size distribution of Carbomix phases is very narrow, 5.0 ± 0.2 for 5 mm and 10.0 ± 0.2 for 10 mm respectively, as shown in Figure 2. This mono-dispersed particle size distribution guarantees to offer high efficiency and high resolution separations. Figure 3 shows that a Carbomix Ca-NP5 column provides higher efficiencies for separating monosaccharides as a comparison to other brands.
Various Ionic Forms. The wide range ionic forms of Carbomix phases available allows for most suitable column choices for achieving the best separation for different kinds of sugar molecules, sugar alcohols and other water soluble compounds from different sources (Table 1). For instance, arabinose, ribitol and arabitol are difficult to be separated on an H-form phase but well resolved on a Ca-form column; the peaks of xylose, galactose, and mannose on Ca-form column merge to one but elutes separately one after another on a Pb-form column.
 
Low Crosslinkage. The low crosslinking property of Carbomix resins allows for proper swell in the mobile, especially at high temperature that is a typical operation for fulfilling most separation tasks. Such swelling effect results in optimized surface area, permeability, capacity, selectivity, and response to changes in ionic strength for separation. The lower the crosslinkage of PS/DVB beads, the more open the phase structure and the permeability it is to sample like larger oligosaccharides. Figure 4 shows that hexasaccharide (G6) is well separated from the sample matrix on a 5% Carbomix Ca-NP5 column accompanied by two more peaks, heptasaccharide (G7) and octasaccharide (G8); however, on an 8% Carbomix Ca-NP5 column, not only G7 and G8 peaks cannot be observed, but also G6 peak is hard to be distinguished.
Stability. Carbomix HPLC columns are well manufactured with proprietary packing technique to guarantee high stability. Carbomix resins are stable in pure water and other aqueous buffers at elevated temperature. As shown in Figure 5, the retention time of the acids changed only 0.1% after continuously washed with 578 column volumes at 55℃ for 6 days. The robustness of Carbomix phase is also confirmed by its reproducibility. Figure 6 shows the consistent retention time, only 0.1% for a Carbomix Ca-NP5 column.
Column Configuration. Carbomix resins can be packed into wide range of column dimensions with ID from 75 mm to 21.2 mm and the length from 5 cm to 30 cm. Custom columns are also available. Column length and diameter affect resolution and analysis time. The principle for choosing a suitable column is to use only as much resin as needed to achieve the desired separation.
The partition process on Carbomix phases is moderated by the counterion (H+, Ca2+, Pb2+, K+ and Na+) bounded to the surface. Usually, at least two or more mechanism, including size-exclusion, ion-exclusion, ion-exchange, ligand-exchange, reversed-phase and normal-phase, are involved. As shown in Figure 4, size-exclusion mechanism is the primary one for oligosaccharides. However, ligand-exchange is the primary one for separating monosaccharides, e.g., the separation of a- and b-anomers of glucose on a Ca-form phase. To fulfill the separation of a molasses sample, the first primary mechanism involved is ion-exclusion, which allows inorganic sodium to be eluted near the void volume. And then size-exclusion and ligand-exchange take effect one after the other for oligosaccharides and monosaccharides. Ion-exclusion can still play a role for sugar alcohols and carboxylic acids in the matrix. As to Carbomix-H-form phase for the separation of organic acids, both reversed-phase and ion-exclusion are the primary mechanism. Hydrophobicity and pKa together impact the retention time of a component in the sample. It is due to this property, it has turned Carbomix-H phase into an ideal choice for monitoring changing components in the process of fermentation.
|
Compound
|
Carbomix H-NP5
|
Carbomix H-NP10
|
Carbomix Ca-NP10
|
|
8%
|
5%
|
8%
|
10%
|
8%
|
10%
|
|
Acetic acid
|
16.07
|
17.52
|
14.64
|
13.39
|
/
|
/
|
|
Adonitol
|
12.15
|
13.59
|
11.10
|
10.26
|
14.73
|
13.67
|
|
Arabinitol
|
12.33
|
13.82
|
11.30
|
10.41
|
17.72
|
16.06
|
|
D-(-)-Arabinose
|
11.90
|
13.46
|
10.93
|
10.08
|
13.43
|
12.52
|
|
L-(+)-Arabinose
|
11.89
|
13.45
|
10.93
|
10.08
|
13.41
|
12.53
|
|
1,4-Butanediol
|
23.14
|
23.11
|
/
|
19.26
|
15.96
|
14.48
|
|
n-Butanol
|
/
|
35.93
|
/
|
32.66
|
/
|
/
|
|
sec-butyl alcohol
|
33.91
|
31.90
|
30.25
|
28.23
|
21.06
|
19.01
|
|
D-(+)-Cellobiose
|
9.41
|
9.79
|
8.18
|
8.17
|
8.81
|
8.96
|
|
Citric acid
|
9.63
|
10.26
|
8.69
|
8.35
|
/
|
/
|
|
Erythriyol
|
13.16
|
14.70
|
11.94
|
11.00
|
15.98
|
14.47
|
|
Ethanol
|
25.07
|
23.13
|
21.82
|
20.72
|
16.38
|
15.89
|
|
Formic acid
|
14.97
|
16.11
|
13.51
|
12.48
|
/
|
/
|
|
D-Fructose
|
11.24
|
12.65
|
10.27
|
9.58
|
13.34
|
12.45
|
|
Fumaric acid
|
13.08
|
7.37
|
7.16
|
7.49
|
/
|
/
|
|
Galactitol
|
11.61
|
13.13
|
10.66
|
9.87
|
19.44
|
18.05
|
|
D-(+)-Galactose
|
11.16
|
12.54
|
10.15
|
9.48
|
11.77
|
11.20
|
|
Glycerol
|
14.89
|
16.07
|
13.61
|
12.46
|
16.20
|
15.12
|
|
D-Glucose
|
10.73
|
11.90
|
9.68
|
9.16
|
10.61
|
10.32
|
|
IPA
|
27.12
|
25.79
|
23.89
|
22.53
|
17.16
|
15.88
|
|
Lactic acid
|
13.66
|
15.41
|
12.70
|
11.55
|
/
|
/
|
|
B-Lactose
|
9.60
|
10.24
|
8.42
|
8.29
|
9.25
|
9.22
|
|
D-Lyxose
|
11.62
|
13.08
|
10.64
|
9.87
|
13.96
|
13.02
|
|
Maleic acid
|
10.01
|
9.50
|
8.53
|
8.56
|
/
|
/
|
|
Malic acid
|
8.66
|
12.03
|
9.80
|
9.24
|
/
|
/
|
|
Maltitol
|
9.72
|
10.51
|
8.41
|
8.29
|
11.92
|
11.24
|
|
D-(+)-Maltose
|
9.51
|
10.01
|
8.29
|
8.23
|
9.02
|
9.09
|
|
Maltotriose
|
9.08
|
8.94
|
7.70
|
7.90
|
8.33
|
8.57
|
|
D-Mannitol
|
11.56
|
12.99
|
10.53
|
9.79
|
17.34
|
15.60
|
|
D-(+)-Mannose
|
11.13
|
12.55
|
10.13
|
9.48
|
12.05
|
11.45
|
|
D-(+)-Melezitose
|
/
|
/
|
/
|
/
|
8.18
|
8.49
|
|
Methanol
|
22.17
|
20.60
|
19.21
|
18.28
|
16.08
|
15.64
|
|
Oxalic acid dihydrate
|
8.94
|
7.72
|
7.44
|
7.72
|
/
|
/
|
|
1-Propanol
|
30.00
|
27.83
|
26.56
|
25.16
|
20.44
|
18.95
|
|
Pyruvic acid
|
10.69
|
10.72
|
10.37
|
9.24
|
/
|
/
|
|
D-(-)-Ribose
|
12.09
|
13.73
|
11.16
|
10.25
|
20.70
|
19.23
|
|
D-Sorbitol
|
11.61
|
13.12
|
10.64
|
9.86
|
20.22
|
18.71
|
|
Succinic acid
|
12.26
|
14.45
|
11.54
|
10.47
|
/
|
/
|
|
D-(+)-Sucrose
|
/
|
/
|
/
|
/
|
8.93
|
9.03
|
|
Tartartic
|
10.00
|
10.74
|
8.94
|
8.64
|
/
|
/
|
|
Triethylene glycol
|
16.92
|
19.60
|
16.12
|
14.17
|
17.51
|
17.21
|
|
Xylitol
|
12.46
|
14.03
|
11.47
|
10.53
|
21.08
|
18.66
|
|
D-Xylose
|
11.32
|
12.61
|
10.24
|
9.60
|
11.63
|
11.19
|
Available Phases
| Part No |
Description |
Price |
| 251005-4605 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 251005-4630 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 251005-7805 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 251005-7830 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 251005-10030 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 251005-21230 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 251008-4605 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 251008-4630 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 251008-7805 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 251008-7830 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 251008-10030 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 251008-21230 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 251010-4605 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 251010-4630 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 251010-7805 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 251010-7830 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 251010-10030 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 251010-21230 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
|
| 250508-4605 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 50 x 4.6 mm |
Enquire |
| 250508-4630 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 4.6 mm |
Enquire |
| 250508-7805 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 50 x 7.8 mm |
Enquire |
| 250508-7830 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 7.8 mm |
Enquire |
| 250508-10030 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 10 mm |
Enquire |
| 250508-21230 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 21.2 mm |
Enquire |
| Clcik Here |
|
| Part No |
Full Description |
Price |
| 261005-4605 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 261005-4630 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 261005-7805 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 261005-7830 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 261005-10030 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 261005-21230 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 261008-4605 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 261008-4630 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 261008-7805 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 261008-7830 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 261008-10030 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 261008-21230 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 261010-4605 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 261010-4630 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 261010-7805 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 261010-7830 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 261010-10030 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 261010-21230 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 260508-4605 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 50 x 4.6 mm |
Enquire |
| 260508-4630 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 4.6 mm |
Enquire |
| 260508-7805 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 50 x 7.8 mm |
Enquire |
| 260508-7830 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 7.8 mm |
Enquire |
| 260508-10030 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 10 mm |
Enquire |
| 260508-21230 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 21.2 mm |
Enquire |
| blank |
| Part No |
Description |
Price |
| 231005-4605 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 231005-4630 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 231005-7805 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 231005-7830 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 231005-10030 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 231005-21230 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 231008-4605 |
Carbomix K-NP10, 8% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 231008-4630 |
Carbomix K-NP10, 8% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 231008-7805 |
Carbomix K-NP10, 8% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 231008-7830 |
Carbomix K-NP10, 8% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 231008-10030 |
Carbomix K-NP10, 8% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 231008-21230 |
Carbomix K-NP10, 8% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
| 231010-4605 |
Carbomix K-NP10, 10% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 231010-4630 |
Carbomix K-NP10, 10% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 231010-7805 |
Carbomix K-NP10, 10% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 231010-7830 |
Carbomix K-NP10, 10% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 231010-10030 |
Carbomix K-NP10, 10% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 231010-21230 |
Carbomix K-NP10, 10% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 230508-4605 |
Carbomix K-NP5, 8% Crosslinkage, 5um, NP 50 x 4.6 mm |
Enquire |
| 230508-4630 |
Carbomix K-NP5, 8% Crosslinkage, 5um, NP 300 x 4.6 mm |
Enquire |
| 230508-7805 |
Carbomix K-NP5, 8% Crosslinkage, 5um, NP 50 x 7.8 mm |
Enquire |
| 230508-7830 |
Carbomix K-NP5, 8% Crosslinkage, 5um, NP 300 x 7.8 mm |
Enquire |
| 230508-10030 |
Carbomix K-NP5, 8% Crosslinkage, 5um, NP 300 x 10 mm |
Enquire |
| 230508-21230 |
Carbomix K-NP5, 8% Crosslinkage, 5um, NP 300 x 21.2 mm |
Enquire |
| blank |
| Part No |
Full Description |
Price |
| 221005-10030 |
Carbomix Na-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 221005-21230 |
Carbomix Na-NP10, 5% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| 221005-4605 |
Carbomix Na-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 221005-4630 |
Carbomix Na-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 221005-7805 |
Carbomix Na-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 221005-7830 |
Carbomix Na-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| blank |
| 221008-4605 |
Carbomix Na-NP10, 8% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 221008-4630 |
Carbomix Na-NP10, 8% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 221008-7805 |
Carbomix Na-NP10, 8% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 221008-7830 |
Carbomix Na-NP10, 8% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 221008-10030 |
Carbomix Na-NP10, 8% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 221008-21230 |
Carbomix Na-NP10, 8% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
| 221010-4605 |
Carbomix Na-NP10, 10% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 221010-4630 |
Carbomix Na-NP10, 10% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 221010-7805 |
Carbomix Na-NP10, 10% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 221010-7830 |
Carbomix Na-NP10, 10% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 221010-10030 |
Carbomix Na-NP10, 10% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 221010-21230 |
Carbomix Na-NP10, 10% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
| 220508-4605 |
Carbomix Na-NP5, 8% Crosslinkage, 5um, NP 50 x 4.6 mm |
Enquire |
| 220508-4630 |
Carbomix Na-NP5, 8% Crosslinkage, 5um, NP 300 x 4.6 mm |
Enquire |
| 220508-7805 |
Carbomix Na-NP5, 8% Crosslinkage, 5um, NP 50 x 7.8 mm |
Enquire |
| 220508-7830 |
Carbomix Na-NP5, 8% Crosslinkage, 5um, NP 300 x 7.8 mm |
Enquire |
| 220508-10030 |
Carbomix Na-NP5, 8% Crosslinkage, 5um, NP 300 x 10 mm |
Enquire |
| 220508-21230 |
Carbomix Na-NP5, 8% Crosslinkage, 5um, NP 300 x 21.2 mm |
Enquire |
| blank |
| Part No |
Description |
Price |
| 241005-4605 |
Carbomix Pb-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 241005-4630 |
Carbomix Pb-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 241005-7805 |
Carbomix Pb-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 241005-7830 |
Carbomix Pb-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 241005-10030 |
Carbomix Pb-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 241005-21230 |
Carbomix Pb-NP10, 5% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
| 241008-4605 |
Carbomix Pb-NP10, 8% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 241008-4630 |
Carbomix Pb-NP10, 8% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 241008-7805 |
Carbomix Pb-NP10, 8% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 241008-7830 |
Carbomix Pb-NP10, 8% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 241008-10030 |
Carbomix Pb-NP10, 8% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 241008-21230 |
Carbomix Pb-NP10, 8% Crosslinkage, 10um, NP 300 x 21.2 mm |
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| blank |
| 241010-4605 |
Carbomix Pb-NP10, 10% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 241010-4630 |
Carbomix Pb-NP10, 10% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 241010-7805 |
Carbomix Pb-NP10, 10% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 241010-7830 |
Carbomix Pb-NP10, 10% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 241010-10030 |
Carbomix Pb-NP10, 10% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 241010-21230 |
Carbomix Pb-NP10, 10% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 240508-4605 |
Carbomix Pb-NP5, 8% Crosslinkage, 5um, NP 50 x 4.6 mm |
Enquire |
| 240508-4630 |
Carbomix Pb-NP5, 8% Crosslinkage, 5um, NP 300 x 4.6 mm |
Enquire |
| 240508-7805 |
Carbomix Pb-NP5, 8% Crosslinkage, 5um, NP 50 x 7.8 mm |
Enquire |
| 240508-7830 |
Carbomix Pb-NP5, 8% Crosslinkage, 5um, NP 300 x 7.8 mm |
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| 240508-10030 |
Carbomix Pb-NP5, 8% Crosslinkage, 5um, NP 300 x 10 mm |
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| 240508-21230 |
Carbomix Pb-NP5, 8% Crosslinkage, 5um, NP 300 x 21.2 mm |
Enquire |
Carbomix
Carbomix lines of columns have been specifically designed for high resolution separation of water soluble and partially water soluble organic compounds, including carbohydrates, organic acids, peptides, and small bio organic molecules involved in cell metabolism. These novel packing materials are based on low crosslinked (5%, 8% and 10%) polystyrene/divinylbenzene (PS/DVB) particles (5 and 10 mm) with surface modification of sulfonic acid (-SO3H) for Carbomix H-NP resins, followed by chelating of various metal ions, calcium ions (Ca2+) for Carbomix Ca-NP, lead ion (Pb2+) for Carbomix Pb-NP, potassium ion (K+) for Carbomix K-NP, and sodium ion (Na+) for Carbomix Na-NP resins (Figure 1).
Uniform Particle size. The particle size distribution of Carbomix phases is very narrow, 5.0 ± 0.2 for 5 mm and 10.0 ± 0.2 for 10 mm respectively, as shown in Figure 2. This mono-dispersed particle size distribution guarantees to offer high efficiency and high resolution separations. Figure 3 shows that a Carbomix Ca-NP5 column provides higher efficiencies for separating monosaccharides as a comparison to other brands.
Various Ionic Forms. The wide range ionic forms of Carbomix phases available allows for most suitable column choices for achieving the best separation for different kinds of sugar molecules, sugar alcohols and other water soluble compounds from different sources (Table 1). For instance, arabinose, ribitol and arabitol are difficult to be separated on an H-form phase but well resolved on a Ca-form column; the peaks of xylose, galactose, and mannose on Ca-form column merge to one but elutes separately one after another on a Pb-form column.
Low Crosslinkage. The low crosslinking property of Carbomix resins allows for proper swell in the mobile, especially at high temperature that is a typical operation for fulfilling most separation tasks. Such swelling effect results in optimized surface area, permeability, capacity, selectivity, and response to changes in ionic strength for separation. The lower the crosslinkage of PS/DVB beads, the more open the phase structure and the permeability it is to sample like larger oligosaccharides. Figure 4 shows that hexasaccharide (G6) is well separated from the sample matrix on a 5% Carbomix Ca-NP5 column accompanied by two more peaks, heptasaccharide (G7) and octasaccharide (G8); however, on an 8% Carbomix Ca-NP5 column, not only G7 and G8 peaks cannot be observed, but also G6 peak is hard to be distinguished.
Stability. Carbomix HPLC columns are well manufactured with proprietary packing technique to guarantee high stability. Carbomix resins are stable in pure water and other aqueous buffers at elevated temperature. As shown in Figure 5, the retention time of the acids changed only 0.1% after continuously washed with 578 column volumes at 55℃ for 6 days. The robustness of Carbomix phase is also confirmed by its reproducibility. Figure 6 shows the consistent retention time, only 0.1% for a Carbomix Ca-NP5 column.
Column Configuration. Carbomix resins can be packed into wide range of column dimensions with ID from 75 mm to 21.2 mm and the length from 5 cm to 30 cm. Custom columns are also available. Column length and diameter affect resolution and analysis time. The principle for choosing a suitable column is to use only as much resin as needed to achieve the desired separation.
The partition process on Carbomix phases is moderated by the counterion (H+, Ca2+, Pb2+, K+ and Na+) bounded to the surface. Usually, at least two or more mechanism, including size-exclusion, ion-exclusion, ion-exchange, ligand-exchange, reversed-phase and normal-phase, are involved. As shown in Figure 4, size-exclusion mechanism is the primary one for oligosaccharides. However, ligand-exchange is the primary one for separating monosaccharides, e.g., the separation of a- and b-anomers of glucose on a Ca-form phase. To fulfill the separation of a molasses sample, the first primary mechanism involved is ion-exclusion, which allows inorganic sodium to be eluted near the void volume. And then size-exclusion and ligand-exchange take effect one after the other for oligosaccharides and monosaccharides. Ion-exclusion can still play a role for sugar alcohols and carboxylic acids in the matrix. As to Carbomix-H-form phase for the separation of organic acids, both reversed-phase and ion-exclusion are the primary mechanism. Hydrophobicity and pKa together impact the retention time of a component in the sample. It is due to this property, it has turned Carbomix-H phase into an ideal choice for monitoring changing components in the process of fermentation.
|
Compound
|
Carbomix H-NP5
|
Carbomix H-NP10
|
Carbomix Ca-NP10
|
|
8%
|
5%
|
8%
|
10%
|
8%
|
10%
|
|
Acetic acid
|
16.07
|
17.52
|
14.64
|
13.39
|
/
|
/
|
|
Adonitol
|
12.15
|
13.59
|
11.10
|
10.26
|
14.73
|
13.67
|
|
Arabinitol
|
12.33
|
13.82
|
11.30
|
10.41
|
17.72
|
16.06
|
|
D-(-)-Arabinose
|
11.90
|
13.46
|
10.93
|
10.08
|
13.43
|
12.52
|
|
L-(+)-Arabinose
|
11.89
|
13.45
|
10.93
|
10.08
|
13.41
|
12.53
|
|
1,4-Butanediol
|
23.14
|
23.11
|
/
|
19.26
|
15.96
|
14.48
|
|
n-Butanol
|
/
|
35.93
|
/
|
32.66
|
/
|
/
|
|
sec-butyl alcohol
|
33.91
|
31.90
|
30.25
|
28.23
|
21.06
|
19.01
|
|
D-(+)-Cellobiose
|
9.41
|
9.79
|
8.18
|
8.17
|
8.81
|
8.96
|
|
Citric acid
|
9.63
|
10.26
|
8.69
|
8.35
|
/
|
/
|
|
Erythriyol
|
13.16
|
14.70
|
11.94
|
11.00
|
15.98
|
14.47
|
|
Ethanol
|
25.07
|
23.13
|
21.82
|
20.72
|
16.38
|
15.89
|
|
Formic acid
|
14.97
|
16.11
|
13.51
|
12.48
|
/
|
/
|
|
D-Fructose
|
11.24
|
12.65
|
10.27
|
9.58
|
13.34
|
12.45
|
|
Fumaric acid
|
13.08
|
7.37
|
7.16
|
7.49
|
/
|
/
|
|
Galactitol
|
11.61
|
13.13
|
10.66
|
9.87
|
19.44
|
18.05
|
|
D-(+)-Galactose
|
11.16
|
12.54
|
10.15
|
9.48
|
11.77
|
11.20
|
|
Glycerol
|
14.89
|
16.07
|
13.61
|
12.46
|
16.20
|
15.12
|
|
D-Glucose
|
10.73
|
11.90
|
9.68
|
9.16
|
10.61
|
10.32
|
|
IPA
|
27.12
|
25.79
|
23.89
|
22.53
|
17.16
|
15.88
|
|
Lactic acid
|
13.66
|
15.41
|
12.70
|
11.55
|
/
|
/
|
|
B-Lactose
|
9.60
|
10.24
|
8.42
|
8.29
|
9.25
|
9.22
|
|
D-Lyxose
|
11.62
|
13.08
|
10.64
|
9.87
|
13.96
|
13.02
|
|
Maleic acid
|
10.01
|
9.50
|
8.53
|
8.56
|
/
|
/
|
|
Malic acid
|
8.66
|
12.03
|
9.80
|
9.24
|
/
|
/
|
|
Maltitol
|
9.72
|
10.51
|
8.41
|
8.29
|
11.92
|
11.24
|
|
D-(+)-Maltose
|
9.51
|
10.01
|
8.29
|
8.23
|
9.02
|
9.09
|
|
Maltotriose
|
9.08
|
8.94
|
7.70
|
7.90
|
8.33
|
8.57
|
|
D-Mannitol
|
11.56
|
12.99
|
10.53
|
9.79
|
17.34
|
15.60
|
|
D-(+)-Mannose
|
11.13
|
12.55
|
10.13
|
9.48
|
12.05
|
11.45
|
|
D-(+)-Melezitose
|
/
|
/
|
/
|
/
|
8.18
|
8.49
|
|
Methanol
|
22.17
|
20.60
|
19.21
|
18.28
|
16.08
|
15.64
|
|
Oxalic acid dihydrate
|
8.94
|
7.72
|
7.44
|
7.72
|
/
|
/
|
|
1-Propanol
|
30.00
|
27.83
|
26.56
|
25.16
|
20.44
|
18.95
|
|
Pyruvic acid
|
10.69
|
10.72
|
10.37
|
9.24
|
/
|
/
|
|
D-(-)-Ribose
|
12.09
|
13.73
|
11.16
|
10.25
|
20.70
|
19.23
|
|
D-Sorbitol
|
11.61
|
13.12
|
10.64
|
9.86
|
20.22
|
18.71
|
|
Succinic acid
|
12.26
|
14.45
|
11.54
|
10.47
|
/
|
/
|
|
D-(+)-Sucrose
|
/
|
/
|
/
|
/
|
8.93
|
9.03
|
|
Tartartic
|
10.00
|
10.74
|
8.94
|
8.64
|
/
|
/
|
|
Triethylene glycol
|
16.92
|
19.60
|
16.12
|
14.17
|
17.51
|
17.21
|
|
Xylitol
|
12.46
|
14.03
|
11.47
|
10.53
|
21.08
|
18.66
|
|
D-Xylose
|
11.32
|
12.61
|
10.24
|
9.60
|
11.63
|
11.19
|
Available Phases
| Part No |
Description |
Price |
| 251005-4605 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 251005-4630 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 251005-7805 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 251005-7830 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 251005-10030 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 251005-21230 |
Carbomix Ca-NP10, 5% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 251008-4605 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 251008-4630 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 251008-7805 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 251008-7830 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 251008-10030 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 251008-21230 |
Carbomix Ca-NP10, 8% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 251010-4605 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 251010-4630 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 251010-7805 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 251010-7830 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 251010-10030 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 251010-21230 |
Carbomix Ca-NP10, 10% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
|
| 250508-4605 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 50 x 4.6 mm |
Enquire |
| 250508-4630 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 4.6 mm |
Enquire |
| 250508-7805 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 50 x 7.8 mm |
Enquire |
| 250508-7830 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 7.8 mm |
Enquire |
| 250508-10030 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 10 mm |
Enquire |
| 250508-21230 |
Carbomix Ca-NP5, 8% Crosslinkage, 5um, NP 300 x 21.2 mm |
Enquire |
| Clcik Here |
|
| Part No |
Full Description |
Price |
| 261005-4605 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 261005-4630 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 261005-7805 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 261005-7830 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 261005-10030 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 261005-21230 |
Carbomix H-NP10, 5% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 261008-4605 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 261008-4630 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 261008-7805 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 261008-7830 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 261008-10030 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 261008-21230 |
Carbomix H-NP10, 8% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 261010-4605 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 261010-4630 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 261010-7805 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 261010-7830 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 261010-10030 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 261010-21230 |
Carbomix H-NP10, 10% Crosslinkage, 10um, NP 300 x 21.2 mm |
Enquire |
| blank |
|
| 260508-4605 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 50 x 4.6 mm |
Enquire |
| 260508-4630 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 4.6 mm |
Enquire |
| 260508-7805 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 50 x 7.8 mm |
Enquire |
| 260508-7830 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 7.8 mm |
Enquire |
| 260508-10030 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 10 mm |
Enquire |
| 260508-21230 |
Carbomix H-NP5, 8% Crosslinkage, 5um, NP 300 x 21.2 mm |
Enquire |
| blank |
| Part No |
Description |
Price |
| 231005-4605 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 50 x 4.6 mm |
Enquire |
| 231005-4630 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 300 x 4.6 mm |
Enquire |
| 231005-7805 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 50 x 7.8 mm |
Enquire |
| 231005-7830 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 300 x 7.8 mm |
Enquire |
| 231005-10030 |
Carbomix K-NP10, 5% Crosslinkage, 10um, NP 300 x 10 mm |
Enquire |
| 231005-21230 |
| |